ABSTRACT
Objective:To evaluate the effect of 2-aminoethoxydiphenyl-borate (2-APB) on cardiac dysfunction in arsenic poisoning rats by stratified strain technique.Methods:Thirty-two 12-week-old SD rats were randomly divided into control group ( n=8) and arsenic exposure group ( n=24). After 12 weeks of arsenic exposure, arsenic exposure group was divided into three groups: natural recovery group ( n=8), low-dose intervention group (n=8) and high-dose intervention group ( n=8). The rats were treated with 2-APB for 21 days. After the last administration, the routine parameters and the layered strain parameters were measured by ultrasonic instrument. Then the rats were killed and their blood and myocardial samples were obtained. The levels of serum creatine kinase isoenzyme (CK-MB) and lactate dehydrogenase (LDH) were tested, and the morphological changes of cardiomyocytes were observed by HE staining. Results:Left ventricular ejection fraction (LVEF), left ventricular short axis shortening (LVFS), global circumferential strain of subendocardial myocardium (GCS-endo), global circumferential strain of middle myocardium (GCS-mid), global circumferential strain of subepicardial myocardium (GCS-epi) and circumferential strain rate of left ventricular segments (SrC) in the natural recovery group were lower than those in the control group ( P<0.05), while serum CK-MB and LDH were higher than those in the control group ( P<0.05). Some ultrasonic parameters and biochemical indexes in the low and high dose intervention groups were improved in varying degrees compared with the natural recovery group ( P<0.05). The correlation between GCS-endo and CK-MB was the highest ( r=-0.931, P<0.05). Myocardial HE staining showed that the degree of myocardial cell swelling and necrosis were reduced, and erythrocyte exudation was reduced in the low and high dose intervention groups compared with the natural recovery group. Conclusions:The stratified strain technique can be used to evaluate the protective effect of 2-APB on cardiac dysfunction in arsenic poisoning rats, and GCS-endo may be one of its sensitive indexes.
ABSTRACT
Objective: To explore the application value of three-dimensional speckle tracking imaging (3D-STI) in evaluation on changes of left ventricular systolic function caused by Paclitaxel combined with Carboplatin for treatment of ovarian cancer. Methods: Thirty patients with pathologically diagnosed ovarian cancer (ovarian cancer group) were selected and treated with Paclitaxel combined with Carboplatin chemotherapy. 3D-STI examination was performed before chemotherapy and at the end of 3 and 6 cycles of chemotherapy to obtain left ventricular end diastolic volume (LVEDV), left ventricular end systolic volume (LVESV), left ventricular ejection fraction (LVEF), spherical index (SPI), left ventricular end diastolic mass (LVEDmass), left ventricular end systolic mass (LVESmass), left ventricular global longitudinal strain (GLS), global circumferential strain (GCS), global radial strain (GRS) and global area strain (GAS), and the indexes compared with those of 30 healthy volunteers (control group). ROC curve was used to analyze the diagnostic efficacy of various strain parameters for cardiac function abnormalities in patients with ovarian cancer after chemotherapy. Results: Compared with control group, LVEDV and LVEF decreased, LVEDmass and LVESmass increased, and left ventricular GLS, GCS, GRS and GAS decreased after 3 and 6 cycles of chemotherapy in ovarian cancer group (all P<0.05). ROC curve of systolic peak strain parameters for diagnosis of cardiac function in patients with ovarian cancer after chemotherapy showed that AUC (0.944) and specificity (96.67%) of GAS were the highest at the end of 3 cycles of chemotherapy while GLS and GCS had the highest sensitivity (90.00%).At the end of 6 cycles of chemotherapy, GAS had the highest AUC (0.953) and specificity (96.67%), and GLS had the highest sensitivity (93.33%). Conclusion: 3D-STI can early detect of left ventricular systolic function in patients with ovarian cancer treated with Paclitaxel combined with Carboplatin.